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rat monoclonal anti 1d4b lamp1  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank rat monoclonal anti 1d4b lamp1
    ( A ) Schematic of cytoplasmic compartments and condensates and respective markers in parentheses that were assayed to colocalize with cytoplasmic POLK. ( B ) Cytoplasmic POLK (green) expression colocalizing with G3BP1 and <t>LAMP1</t> (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows POLK colocalizing with LAMP1 and G3BP1. ( C – F ) Cytoplasmic POLK (green), EEA1, CTSB, CTSD, and GBA1 (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows level of POLK colocalization with the proteins. Highest colocalization with CTSD, partial with EEA1 and CTSB, and minimal GBA1.
    Rat Monoclonal Anti 1d4b Lamp1, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 201 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+anti+lamp1/anti-LAMP-1/pmc13171111-80-2-7
    Average 96 stars, based on 201 article reviews
    rat monoclonal anti 1d4b lamp1 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "An altered cell-specific subcellular distribution of translesion synthesis DNA polymerase kappa (POLK) in aging mouse neurons"

    Article Title: An altered cell-specific subcellular distribution of translesion synthesis DNA polymerase kappa (POLK) in aging mouse neurons

    Journal: eLife

    doi: 10.7554/eLife.101533

    ( A ) Schematic of cytoplasmic compartments and condensates and respective markers in parentheses that were assayed to colocalize with cytoplasmic POLK. ( B ) Cytoplasmic POLK (green) expression colocalizing with G3BP1 and LAMP1 (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows POLK colocalizing with LAMP1 and G3BP1. ( C – F ) Cytoplasmic POLK (green), EEA1, CTSB, CTSD, and GBA1 (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows level of POLK colocalization with the proteins. Highest colocalization with CTSD, partial with EEA1 and CTSB, and minimal GBA1.
    Figure Legend Snippet: ( A ) Schematic of cytoplasmic compartments and condensates and respective markers in parentheses that were assayed to colocalize with cytoplasmic POLK. ( B ) Cytoplasmic POLK (green) expression colocalizing with G3BP1 and LAMP1 (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows POLK colocalizing with LAMP1 and G3BP1. ( C – F ) Cytoplasmic POLK (green), EEA1, CTSB, CTSD, and GBA1 (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows level of POLK colocalization with the proteins. Highest colocalization with CTSD, partial with EEA1 and CTSB, and minimal GBA1.

    Techniques Used: Expressing, Staining

    ( A ) Cytoplasmic POLK (green) expression colocalizing with G3BP1 (blue) in fluorescent-nissl stained cells (purple) of mouse brain tissue from M1 and S1 cortical regions in 18-month-old brain but not in young 1 month. ( B ) Cytoplasmic POLK (green) expression colocalizing with LAMP1 (blue) in fluorescent-nissl stained cells (purple) of mouse brain tissue from M1 and S1 cortical regions of 18-month brain. ( C ) Additional representative image with channel separation for immunofluorescence staining of wild-type mouse brain cortical areas S1, showing cytoplasmic POLK is colocalized with stress granule marker G3BP1 and endo/lysosomal marker LAMP1. Arrows indicate few representative sites of colocalization in both images.
    Figure Legend Snippet: ( A ) Cytoplasmic POLK (green) expression colocalizing with G3BP1 (blue) in fluorescent-nissl stained cells (purple) of mouse brain tissue from M1 and S1 cortical regions in 18-month-old brain but not in young 1 month. ( B ) Cytoplasmic POLK (green) expression colocalizing with LAMP1 (blue) in fluorescent-nissl stained cells (purple) of mouse brain tissue from M1 and S1 cortical regions of 18-month brain. ( C ) Additional representative image with channel separation for immunofluorescence staining of wild-type mouse brain cortical areas S1, showing cytoplasmic POLK is colocalized with stress granule marker G3BP1 and endo/lysosomal marker LAMP1. Arrows indicate few representative sites of colocalization in both images.

    Techniques Used: Expressing, Staining, Immunofluorescence, Marker

    Related Articles

    Incubation:

    Article Title: Neuronal subtype governs amyloid structure, cellular response, and cognitive outcome in genetically targeted APP mouse models
    Article Snippet: .. Free-floating sections were washed several times in TBS to remove cryoprotectant and then blocked with TBS containing 0.3% Triton X-100 plus 5–10% normal serum (goat or donkey) for 1 hr at RT before overnight incubation at 4 °C in primary antibodies diluted 1:500 in blocking solution (mouse IgG2a anti-human Aβ clone Ab9 [ ]; rat anti-LAMP1, DSHB #1D4B; rabbit anti-IBA1, Wako #019–19741; mouse IgG1 anti-GFAP, Sigma #G3893; chicken anti-IBA1, Synaptic Systems #234009; and/or rabbit anti-pyroglutamate-3 Aβ, Synaptic Systems #218003). ..

    Article Title: Rab24 protein levels show dynamic changes in mouse tissues and human cancers
    Article Snippet: .. Blocking was performed with 5% normal goat serum in TBS at room temperature for 1 h. Sections were incubated in affinity-purified rabbit anti-Rab24 (11445–1-AP, Proteintech), rabbit anti-LC3 (NB-100-2331, Novus), rabbit anti-Rab5 (sc-28570, Santa Cruz), rabbit anti-Rab7 (9367S, Cell Signaling), rabbit anti-p62/SQSTM1 (AP2183b, Abgent), or a mixture of rat anti-LAMP1 (1D4B, Developmental Studies Hybridoma Bank/DSHB) and rat anti-LAMP2 (ABL-93, DSHB) in 5% normal goat serum at +4 °C overnight (Table ). ..

    Article Title: Rab24 protein levels show dynamic changes in mouse tissues and human cancers.
    Article Snippet: .. Blocking was performed with 5% normal goat serum in TBS at room temperature for 1 h. Sections were incubated in affinitypurified rabbit anti-Rab24 (11445–1-AP, Proteintech), rabbit anti-LC3 (NB-100-2331, Novus), rabbit anti-Rab5 (sc-28570, Santa Cruz), rabbit anti-Rab7 (9367S, Cell Signaling), rabbit anti-p62/SQSTM1 (AP2183b, Abgent), or a mixture of rat anti-LAMP1 (1D4B, Developmental Studies Hybridoma Bank/DSHB) and rat anti-LAMP2 (ABL-93, DSHB) in 5% normal goat serum at +4 °C overnight (Table S1). ..

    Blocking Assay:

    Article Title: Neuronal subtype governs amyloid structure, cellular response, and cognitive outcome in genetically targeted APP mouse models
    Article Snippet: .. Free-floating sections were washed several times in TBS to remove cryoprotectant and then blocked with TBS containing 0.3% Triton X-100 plus 5–10% normal serum (goat or donkey) for 1 hr at RT before overnight incubation at 4 °C in primary antibodies diluted 1:500 in blocking solution (mouse IgG2a anti-human Aβ clone Ab9 [ ]; rat anti-LAMP1, DSHB #1D4B; rabbit anti-IBA1, Wako #019–19741; mouse IgG1 anti-GFAP, Sigma #G3893; chicken anti-IBA1, Synaptic Systems #234009; and/or rabbit anti-pyroglutamate-3 Aβ, Synaptic Systems #218003). ..

    Article Title: Rab24 protein levels show dynamic changes in mouse tissues and human cancers
    Article Snippet: .. Blocking was performed with 5% normal goat serum in TBS at room temperature for 1 h. Sections were incubated in affinity-purified rabbit anti-Rab24 (11445–1-AP, Proteintech), rabbit anti-LC3 (NB-100-2331, Novus), rabbit anti-Rab5 (sc-28570, Santa Cruz), rabbit anti-Rab7 (9367S, Cell Signaling), rabbit anti-p62/SQSTM1 (AP2183b, Abgent), or a mixture of rat anti-LAMP1 (1D4B, Developmental Studies Hybridoma Bank/DSHB) and rat anti-LAMP2 (ABL-93, DSHB) in 5% normal goat serum at +4 °C overnight (Table ). ..

    Article Title: Rab24 protein levels show dynamic changes in mouse tissues and human cancers.
    Article Snippet: .. Blocking was performed with 5% normal goat serum in TBS at room temperature for 1 h. Sections were incubated in affinitypurified rabbit anti-Rab24 (11445–1-AP, Proteintech), rabbit anti-LC3 (NB-100-2331, Novus), rabbit anti-Rab5 (sc-28570, Santa Cruz), rabbit anti-Rab7 (9367S, Cell Signaling), rabbit anti-p62/SQSTM1 (AP2183b, Abgent), or a mixture of rat anti-LAMP1 (1D4B, Developmental Studies Hybridoma Bank/DSHB) and rat anti-LAMP2 (ABL-93, DSHB) in 5% normal goat serum at +4 °C overnight (Table S1). ..

    Immunofluorescence:

    Article Title: Multiparity exacerbates Aβ accumulation and promotes cellular senescence in a mouse model of amyloidosis.
    Article Snippet: Proteins were extracted from cells after 3 days of treatment with 200 nM estradiol (E2) (Sigma Aldrich, E2758), 200 nM progesterone (P4) (MCE, HY-N0437), followed by BCA quantification (Thermo Fisher Scientific, A55864) and western blot analysis. .. Antibodies AR TIC LE IN PR ES S 8 The information of primary antibodies used was as follows: rabbit anti-IBA1 (FUJIFILM Wako Chemicals, 019-19741, PRID: AB_839504), goat anti-IBA1 (Novus, NB100-1028, PRID: AB_3148646, 1:500 for immunofluorescence (IF)), rabbit anti-GFAP (Abcam, ab7260, PRID: AB_305808), rat anti-CD68 (BIO-RAD, MCA1957, PRID: AB_3100585), rat anti-LAMP1 (DSHB, clone 1D4B, PRID: AB_2134500), rabbit anti-PU.1 (CST, 2258S, PRID: AB_2186909), rabbit anti-SYNAPTOPHYSIN (Abcam, ab16659, PRID: AB_443419), rabbit anti-PSD-95 (Invitrogen, 51-6900, PRID: AB_2533914), rabbit anti-BACE1 (CST, 5606S, PRID: AB_1903900), rabbit anti-Amyloid Precursor Protein, C-Terminal (Sigma-Aldrich, A8717, PRID: AB_258409), mouse anti-PSD-95 (1:1000; mouse monoclonal, NeuroMab, 75-028, PRID: AB_2292909), rabbit antiFOSB (1:1000; CST, 2251S, PRID: AB_2106903), rabbit anti-SYNAPTOPHYSIN (1:1000 for WB; Abclonal, A6344, PRID: AB_2766946), rabbit anti-BACE1 (rabbit monoclonal, CST, 5606S, PRID: AB_1903900, 1:1000 for WB, 1:500 for IF), chicken anti-MAP2 (1:5000, Abcam, ab5392, PRID: AB_2138153), rabbit anti-Beta-Actin (1:10000; mouse monoclonal; Proteintech, 66009- 1-Ig, PRID: AB_2687938), rabbit anti-β Tubulin (Proteintech, 10094-1-AP, PRID: AB_2210695, 1:5000 for WB) and mouse anti-GAPDH (ProteinTech, 60004-1-Ig, PRID: AB_2107436, 1:10000 for WB). .. The information of secondary antibodies used was as follows: donkey anti-rabbit IgG (H+L) with Alexa FluorTM 488 (Invitrogen, A-21206, PRID: AB_2535729), donkey anti-rabbit IgG (H+L) with Alexa FluorTM 568 (Invitrogen, A10042, PRID: AB_2534017), donkey anti-rabbit IgG (H+L) with Alexa FluorTM 647 (Invitrogen, A-31573, PRID: AB_2536183), donkey anti-goat IgG (H+L) with Alexa FluorTM 488 (Invitrogen, A-11055, PRID: AB_2534102), donkey anti-goat IgG (H+L) with Alexa FluorTM 568 (Invitrogen, A-11057, PRID: AB_2534104), donkey anti-rat IgG (H+L) with Alexa FluorTM 488 (Invitrogen, A48269, PRID: AB_2893137), donkey anti chicken IgY AR TIC LE IN PR ES S 9 (H+L) highly cross adsorbed secondary antibody, Alexa FluorTM 488 (Invitrogen, A78948, PRID: AB_2921070), all diluted by 10% donkey serum in PBS 1:500 for IF.

    Western Blot:

    Article Title: Multiparity exacerbates Aβ accumulation and promotes cellular senescence in a mouse model of amyloidosis.
    Article Snippet: Proteins were extracted from cells after 3 days of treatment with 200 nM estradiol (E2) (Sigma Aldrich, E2758), 200 nM progesterone (P4) (MCE, HY-N0437), followed by BCA quantification (Thermo Fisher Scientific, A55864) and western blot analysis. .. Antibodies AR TIC LE IN PR ES S 8 The information of primary antibodies used was as follows: rabbit anti-IBA1 (FUJIFILM Wako Chemicals, 019-19741, PRID: AB_839504), goat anti-IBA1 (Novus, NB100-1028, PRID: AB_3148646, 1:500 for immunofluorescence (IF)), rabbit anti-GFAP (Abcam, ab7260, PRID: AB_305808), rat anti-CD68 (BIO-RAD, MCA1957, PRID: AB_3100585), rat anti-LAMP1 (DSHB, clone 1D4B, PRID: AB_2134500), rabbit anti-PU.1 (CST, 2258S, PRID: AB_2186909), rabbit anti-SYNAPTOPHYSIN (Abcam, ab16659, PRID: AB_443419), rabbit anti-PSD-95 (Invitrogen, 51-6900, PRID: AB_2533914), rabbit anti-BACE1 (CST, 5606S, PRID: AB_1903900), rabbit anti-Amyloid Precursor Protein, C-Terminal (Sigma-Aldrich, A8717, PRID: AB_258409), mouse anti-PSD-95 (1:1000; mouse monoclonal, NeuroMab, 75-028, PRID: AB_2292909), rabbit antiFOSB (1:1000; CST, 2251S, PRID: AB_2106903), rabbit anti-SYNAPTOPHYSIN (1:1000 for WB; Abclonal, A6344, PRID: AB_2766946), rabbit anti-BACE1 (rabbit monoclonal, CST, 5606S, PRID: AB_1903900, 1:1000 for WB, 1:500 for IF), chicken anti-MAP2 (1:5000, Abcam, ab5392, PRID: AB_2138153), rabbit anti-Beta-Actin (1:10000; mouse monoclonal; Proteintech, 66009- 1-Ig, PRID: AB_2687938), rabbit anti-β Tubulin (Proteintech, 10094-1-AP, PRID: AB_2210695, 1:5000 for WB) and mouse anti-GAPDH (ProteinTech, 60004-1-Ig, PRID: AB_2107436, 1:10000 for WB). .. The information of secondary antibodies used was as follows: donkey anti-rabbit IgG (H+L) with Alexa FluorTM 488 (Invitrogen, A-21206, PRID: AB_2535729), donkey anti-rabbit IgG (H+L) with Alexa FluorTM 568 (Invitrogen, A10042, PRID: AB_2534017), donkey anti-rabbit IgG (H+L) with Alexa FluorTM 647 (Invitrogen, A-31573, PRID: AB_2536183), donkey anti-goat IgG (H+L) with Alexa FluorTM 488 (Invitrogen, A-11055, PRID: AB_2534102), donkey anti-goat IgG (H+L) with Alexa FluorTM 568 (Invitrogen, A-11057, PRID: AB_2534104), donkey anti-rat IgG (H+L) with Alexa FluorTM 488 (Invitrogen, A48269, PRID: AB_2893137), donkey anti chicken IgY AR TIC LE IN PR ES S 9 (H+L) highly cross adsorbed secondary antibody, Alexa FluorTM 488 (Invitrogen, A78948, PRID: AB_2921070), all diluted by 10% donkey serum in PBS 1:500 for IF.

    Immunostaining:

    Article Title: p53 Drives Lung Cancer Regression through a TSC2/TFEB-dependent Senescence Program
    Article Snippet: .. Immunostaining was performed using the following primary antibodies: goat anti-AGER (R&D Systems, cat. #AF1145, RRID: AB_354628; 1:200), rat anti-AGER (R&D Systems, cat. #MAB1179, RRID: AB_2289349; 1:100), rabbit HOPX (Proteintech, cat. #11419-1-AP, RRID: AB_10693525; 1:100), rabbit anti-SPC (Millipore, cat. #AB3786, RRID: AB_91588; 1:100), rabbit anti-p44/42 MAPK (Erk1/2; Cell Signaling Technology, cat. #4370, RRID: AB_2315112; 1:100), rabbit anti-CC3 (Cell Signaling Technology, cat. #9661, RRID: AB_2341188; 1:100), rabbit anti-TFEB (Bethyl Laboratories, cat. #A303-673A, RRID: AB_11204751; 1:100), rat anti-LAMP1 (Developmental Studies Hybridoma Bank (DSHB), cat. #1d4b, RRID: AB_2134500; 1:100), rabbit anti-p53 (Leica Biosystems, cat. #P53-CM5P, RRID: AB_2744683; 1:100), mouse anti-p53 (Cell Signaling Technology, cat. #2524, RRID: AB_331743; 1:100), and mouse anti-BrdU (BD Biosciences, cat. #555627, RRID: AB_395993; 1:50). .. Secondary antibodies used were as follows: goat anti–rat-AF488 (Thermo Fisher Scientific, cat. #A-11006, RRID: AB_2534074), goat anti–rabbit-FITC (Vector Laboratories, cat. #FI-1000, RRID: AB_2336197), horse anti–mouse-FITC (Vector Laboratories, cat. #FI-2000, RRID: AB_2336176), goat anti–mouse-AF546 (Thermo Fisher Scientific, cat. #A-11030, RRID: AB_2737024), goat anti–rabbit-546 (Thermo Fisher Scientific, cat. #A-11035, RRID: AB_2534093), biotinylated goat anti-mouse (Vector Laboratories, cat. #BA-9200, RRID:AB_2336171), biotinylated goat anti-rabbit (Vector Laboratories, cat. #BA-1000, RRID: AB_2313606), and biotinylated horse anti-goat (Vector Laboratories, cat. #BA-9500, RRID: AB_2336123).

    Marker:

    Article Title: Microglial interferon signaling and Aβ plaque pathology are enhanced in female 5xFAD Alzheimer’s disease mice, independent of estrous cycle stage
    Article Snippet: The tissue was then mounted on 2 × subbed slides with Prolong Gold anti-fade mounting media (Invitrogen, #P36930) and cover slipped. .. Primary and secondary antibody dilutions included rabbit anti-Iba1 as a pan-microglial marker (Wako, #019–19741, 1:1000), biotinylated 6E10 (BioLengend, #803,008, 1:3000), rat anti-LAMP1 as a neuritic dystrophy marker (DSHB, Clone 1D4B, 1:2000), goat anti-rabbit Alexa 488 (Invitrogen, #A21245, 1:1500), Streptavidin Alexa 594 (Invitrogen, #S32356, 1:1500), and goat anti-rat Alexa 594 (Invitrogen, #A11007, 1:1000). ..

    Article Title: Microglial interferon signaling and Aβ plaque pathology are enhanced in female 5xFAD Alzheimer's disease mice, independent of estrous cycle stage.
    Article Snippet: The tissue was then mounted on 2x subbed slides with Prolong Gold anti-fade mounting media (Invitrogen, #P36930) and cover slipped. .. Primary and secondary antibody dilutions included rabbit anti-Iba1 as a pan-microglial marker (Wako, #019- 19741, 1:1000), biotinylated 6E10 (BioLengend, #803008, 1:3000), rat anti-LAMP1 as a neuritic dystrophy marker (DSHB, Clone 1D4B, 1:2000), goat anti-rabbit Alexa 488 (Invitrogen, #A21245, 1:1500), Streptavidin Alexa 594 (Invitrogen, #S32356, 1:1500), and goat anti-rat Alexa 594 (Invitrogen, #A11007, 1:1000). ..

    Affinity Purification:

    Article Title: Rab24 protein levels show dynamic changes in mouse tissues and human cancers
    Article Snippet: .. Blocking was performed with 5% normal goat serum in TBS at room temperature for 1 h. Sections were incubated in affinity-purified rabbit anti-Rab24 (11445–1-AP, Proteintech), rabbit anti-LC3 (NB-100-2331, Novus), rabbit anti-Rab5 (sc-28570, Santa Cruz), rabbit anti-Rab7 (9367S, Cell Signaling), rabbit anti-p62/SQSTM1 (AP2183b, Abgent), or a mixture of rat anti-LAMP1 (1D4B, Developmental Studies Hybridoma Bank/DSHB) and rat anti-LAMP2 (ABL-93, DSHB) in 5% normal goat serum at +4 °C overnight (Table ). ..



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    BAITs promote DC activation and antigen presentation via enhanced lysosomal processing. (A) Schematic for MHC Ⅰ/Ⅱ immunostaining. DCs were treated with antigens or BAITs (12 h), then incubated in fresh medium (with TGFβ) for 24 h before staining. (B, C) Quantification of surface MHC Ⅰ (B) and MHC Ⅱ (C) on DCs (n = 8; n.s. is P > 0.05, ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). (D, E) Representative immunofluorescence images of DCs (blue: nuclei; yellow: <t>LAMP1;</t> green: MHC Ⅰ; magenta: MHC Ⅱ). BAIT-treated DCs show strong surface MHC and minimal intracellular antigen signal, whereas antigen-treated DCs show retained antigen (red) and weak surface MHC signal. (F–H) Flow cytometric analysis of MHC Ⅰ (F), MHC Ⅱ (G), and CD80 (H) expression on murine CD11c + DCs after exposure to antigens or BAITs. (I) Quantification of MHC Ⅰ, MHC Ⅱ, and CD80 expression in DCs by flow cytometry (n = 3; ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (J) Heatmap of DC activation-related gene expression after treatment with antigens or BAITs, highlighting upregulation of maturation and antigen presentation genes and downregulation of immunosuppressive genes by BAITs (n = 3). Data are presented as mean ± SD.
    Mab4320 Rat, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rat anti lamp1 1d4b  (Santa Cruz Biotechnology)
    96
    Santa Cruz Biotechnology rat anti lamp1 1d4b
    BAITs promote DC activation and antigen presentation via enhanced lysosomal processing. (A) Schematic for MHC Ⅰ/Ⅱ immunostaining. DCs were treated with antigens or BAITs (12 h), then incubated in fresh medium (with TGFβ) for 24 h before staining. (B, C) Quantification of surface MHC Ⅰ (B) and MHC Ⅱ (C) on DCs (n = 8; n.s. is P > 0.05, ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). (D, E) Representative immunofluorescence images of DCs (blue: nuclei; yellow: <t>LAMP1;</t> green: MHC Ⅰ; magenta: MHC Ⅱ). BAIT-treated DCs show strong surface MHC and minimal intracellular antigen signal, whereas antigen-treated DCs show retained antigen (red) and weak surface MHC signal. (F–H) Flow cytometric analysis of MHC Ⅰ (F), MHC Ⅱ (G), and CD80 (H) expression on murine CD11c + DCs after exposure to antigens or BAITs. (I) Quantification of MHC Ⅰ, MHC Ⅱ, and CD80 expression in DCs by flow cytometry (n = 3; ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (J) Heatmap of DC activation-related gene expression after treatment with antigens or BAITs, highlighting upregulation of maturation and antigen presentation genes and downregulation of immunosuppressive genes by BAITs (n = 3). Data are presented as mean ± SD.
    Rat Anti Lamp1 1d4b, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+anti+lamp1/LAMP-1+Antibody/bio_rxiv__64898__2026__02__13__705687-41-4-8
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    Image Search Results


    ( A ) Schematic of cytoplasmic compartments and condensates and respective markers in parentheses that were assayed to colocalize with cytoplasmic POLK. ( B ) Cytoplasmic POLK (green) expression colocalizing with G3BP1 and LAMP1 (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows POLK colocalizing with LAMP1 and G3BP1. ( C – F ) Cytoplasmic POLK (green), EEA1, CTSB, CTSD, and GBA1 (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows level of POLK colocalization with the proteins. Highest colocalization with CTSD, partial with EEA1 and CTSB, and minimal GBA1.

    Journal: eLife

    Article Title: An altered cell-specific subcellular distribution of translesion synthesis DNA polymerase kappa (POLK) in aging mouse neurons

    doi: 10.7554/eLife.101533

    Figure Lengend Snippet: ( A ) Schematic of cytoplasmic compartments and condensates and respective markers in parentheses that were assayed to colocalize with cytoplasmic POLK. ( B ) Cytoplasmic POLK (green) expression colocalizing with G3BP1 and LAMP1 (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows POLK colocalizing with LAMP1 and G3BP1. ( C – F ) Cytoplasmic POLK (green), EEA1, CTSB, CTSD, and GBA1 (red) in fluorescent-nissl stained cells (blue) of mouse brain tissue in 18-month-old brain. Line scan in the boxed region shows level of POLK colocalization with the proteins. Highest colocalization with CTSD, partial with EEA1 and CTSB, and minimal GBA1.

    Article Snippet: Antibody , Rat monoclonal anti-1D4B (LAMP1) , DSHB , RRID: AB_2134500 , IF (1:200).

    Techniques: Expressing, Staining

    ( A ) Cytoplasmic POLK (green) expression colocalizing with G3BP1 (blue) in fluorescent-nissl stained cells (purple) of mouse brain tissue from M1 and S1 cortical regions in 18-month-old brain but not in young 1 month. ( B ) Cytoplasmic POLK (green) expression colocalizing with LAMP1 (blue) in fluorescent-nissl stained cells (purple) of mouse brain tissue from M1 and S1 cortical regions of 18-month brain. ( C ) Additional representative image with channel separation for immunofluorescence staining of wild-type mouse brain cortical areas S1, showing cytoplasmic POLK is colocalized with stress granule marker G3BP1 and endo/lysosomal marker LAMP1. Arrows indicate few representative sites of colocalization in both images.

    Journal: eLife

    Article Title: An altered cell-specific subcellular distribution of translesion synthesis DNA polymerase kappa (POLK) in aging mouse neurons

    doi: 10.7554/eLife.101533

    Figure Lengend Snippet: ( A ) Cytoplasmic POLK (green) expression colocalizing with G3BP1 (blue) in fluorescent-nissl stained cells (purple) of mouse brain tissue from M1 and S1 cortical regions in 18-month-old brain but not in young 1 month. ( B ) Cytoplasmic POLK (green) expression colocalizing with LAMP1 (blue) in fluorescent-nissl stained cells (purple) of mouse brain tissue from M1 and S1 cortical regions of 18-month brain. ( C ) Additional representative image with channel separation for immunofluorescence staining of wild-type mouse brain cortical areas S1, showing cytoplasmic POLK is colocalized with stress granule marker G3BP1 and endo/lysosomal marker LAMP1. Arrows indicate few representative sites of colocalization in both images.

    Article Snippet: Antibody , Rat monoclonal anti-1D4B (LAMP1) , DSHB , RRID: AB_2134500 , IF (1:200).

    Techniques: Expressing, Staining, Immunofluorescence, Marker

    BAITs promote DC activation and antigen presentation via enhanced lysosomal processing. (A) Schematic for MHC Ⅰ/Ⅱ immunostaining. DCs were treated with antigens or BAITs (12 h), then incubated in fresh medium (with TGFβ) for 24 h before staining. (B, C) Quantification of surface MHC Ⅰ (B) and MHC Ⅱ (C) on DCs (n = 8; n.s. is P > 0.05, ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). (D, E) Representative immunofluorescence images of DCs (blue: nuclei; yellow: LAMP1; green: MHC Ⅰ; magenta: MHC Ⅱ). BAIT-treated DCs show strong surface MHC and minimal intracellular antigen signal, whereas antigen-treated DCs show retained antigen (red) and weak surface MHC signal. (F–H) Flow cytometric analysis of MHC Ⅰ (F), MHC Ⅱ (G), and CD80 (H) expression on murine CD11c + DCs after exposure to antigens or BAITs. (I) Quantification of MHC Ⅰ, MHC Ⅱ, and CD80 expression in DCs by flow cytometry (n = 3; ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (J) Heatmap of DC activation-related gene expression after treatment with antigens or BAITs, highlighting upregulation of maturation and antigen presentation genes and downregulation of immunosuppressive genes by BAITs (n = 3). Data are presented as mean ± SD.

    Journal: Bioactive Materials

    Article Title: Countering postoperative immune suppression with a self-assembling dendritic cell nanovaccine

    doi: 10.1016/j.bioactmat.2026.05.005

    Figure Lengend Snippet: BAITs promote DC activation and antigen presentation via enhanced lysosomal processing. (A) Schematic for MHC Ⅰ/Ⅱ immunostaining. DCs were treated with antigens or BAITs (12 h), then incubated in fresh medium (with TGFβ) for 24 h before staining. (B, C) Quantification of surface MHC Ⅰ (B) and MHC Ⅱ (C) on DCs (n = 8; n.s. is P > 0.05, ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by one-way ANOVA with Bonferroni post-hoc test). (D, E) Representative immunofluorescence images of DCs (blue: nuclei; yellow: LAMP1; green: MHC Ⅰ; magenta: MHC Ⅱ). BAIT-treated DCs show strong surface MHC and minimal intracellular antigen signal, whereas antigen-treated DCs show retained antigen (red) and weak surface MHC signal. (F–H) Flow cytometric analysis of MHC Ⅰ (F), MHC Ⅱ (G), and CD80 (H) expression on murine CD11c + DCs after exposure to antigens or BAITs. (I) Quantification of MHC Ⅰ, MHC Ⅱ, and CD80 expression in DCs by flow cytometry (n = 3; ∗∗∗ is P < 0.001, ∗∗∗∗ is P < 0.0001 by two-way ANOVA with Bonferroni post-hoc test). (J) Heatmap of DC activation-related gene expression after treatment with antigens or BAITs, highlighting upregulation of maturation and antigen presentation genes and downregulation of immunosuppressive genes by BAITs (n = 3). Data are presented as mean ± SD.

    Article Snippet: After treatment, the cells were washed twice with PBS and fixed in 4% paraformaldehyde for 20 min. For intracellular protein staining, fixed cells were permeabilized with 0.1% Triton X-100 at room temperature for 10 min. Next, the cells were blocked with 2% BSA and incubated with rat CoraLite Plus 488 anti-mouse LAMP1 antibody (1:200) and rabbit anti-mouse pSAP (1:200) antibody overnight at 4 °C.

    Techniques: Activation Assay, Immunopeptidomics, Immunostaining, Incubation, Staining, Immunofluorescence, Expressing, Flow Cytometry, Gene Expression